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By F. Sherwood Taylor

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Miro´, TrAC — Trends Anal. , 26 (2007) 18–26. H. Hansen, Quim. , 8 (1989) 139–150. This page intentionally left blank CHAPT ER 2 From Beaker to Programmable Microfluidics Jaromir Ru˚ˇzicˇka Contents 1. 2. 3. 4. 5. 6. 7. 6 Sample pretreatment 8. Conclusions Acknowledgment References 23 26 28 28 35 36 37 37 39 40 41 41 43 43 44 44 1. INTRODUCTION A vast majority of (bio)chemical assays rely on precise and reproducible solution handling, since samples and reagents have to be metered, mixed, incubated, heated, separated, and monitored by spectroscopy, electrochemistry, or other means for quantification of target analytes.

The channel network, which is made by various sophisticated procedures, such as micro-drilling, etching, photolithography, or laser erasing, is impressively exact and reproducible, allowing different channel profiles to be obtained, and it can in many instances be made using inexpensive materials and mass-produced at low cost, in fact, at much lower expenditures than the LOV. , they have a fixed architecture for predetermined chemistries, and to this day operate on a continuous flow basis, mimicking the traditional FIA mode.

This is why the full potentials of FIA were only gradually unravelled as we worked. In 1977 we took the first steps towards gradient techniques with the development of the FIA titration procedure [3], which was soon followed by stopped-flow techniques [4]. Later a number of FIA-gradient techniques were added, notably gradient dilution and calibration, selectivity evaluation methods and gradient scanning — which truly revealed the vast potential of FIA [5,6]. In fact, we came to believe that it was, more than anything else, the gradient techniques, which inherently distinguished FIA from all other continuous flow techniques.

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