Download Alkaline Phosphatase by Robert B. McComb PDF

By Robert B. McComb

ISBN-10: 1461329728

ISBN-13: 9781461329725

There should be doubtless that alkaline phosphatase is among the so much greatly in­ vestigated of all enzymes. This has resulted from the ubiquity of its distribution, and from the benefit and sensitivity with which its job may be measured. regrettably, those wide-ranging yet frequently superficial experimental reports were up by way of in depth and systematic investigations in just a couple of restricted parts of the biochemistry and chemical pathology of alkaline phosphatase. the outcome has been the buildup of a systematic literature of intimidating proportions, and the inevitable rediscovery of already­ recognized proof in regards to the enzyme. Scientists are taught early of their careers that, within the phrases of Sir John Herschel, "Hasty generalization is the bane of technological know-how. " however, moments arrive in all spheres of clinical job whilst generalization turns into crucial, to codify and to choose between the mass of information already collected, and to supply beginning issues for brand spanking new advancements and new traces of research. this is often very true in a box akin to alkaline phosphatase study, within which very actual risks exist that the seeds of basic realizing could be misplaced amidst an unexamined harvest of empirical observations. The heritage of the examine of alkaline phosphatase presents a number of cases whilst worthwhile generalizations have emerged. sometimes, the conclusions drawn at the foundation of obtainable facts have been improper; extra usually, they've got stood the attempt of additional experimentation, and regularly, they've got supplied new insights into the character and correct­ ties of this enzyme.

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18, 215,1914. 110. Bell, R. , and Doisy, E. , Rapid colorimetric methods for the determination of phosphorus in urine and blood, 1. BioI. , 44, 55, 1920. 111. Briggs, A. , A modification of the Bell-Doisy phosphate method, 1. Bioi. , 53, 13, 1922. 112. Kay, H. , Plasma phosphatase. I. Method of determination: Some properties of the enzyme, 1. BioI. , 89,235, 1930. 113. , and Cohen, H. , Micro colorimetric studies. I. A molybdic acid, stannous chloride reagent: The micro estimation of phosphate and calcium in pus, plasma, and spinal fluid, J.

67, 183, 1967. 230. , A new phosphoric ester obtained by the aid of yeast juice; preliminary note, Proc. Chem. , 30, 16, 1914. 231. Neuman, W. , and Neuman, M. , Emerging concepts of the structure and metabolic functions of bone, Am. 1. , 22, 123, 1957. 232. Kugler, O. , Histochemical localization of alkaline phosphatase and nucleic acids in the oocytes of the bluegill (Lepomis machochirus Rajinesque), J. Histochem. , 1,296, 1953. 233. Dempsey, E. , and Wislocki, G. , Histochemical reactions associated with basophilia and acidophilia in the placenta and pituitary gland, Am.

1). Indeed, Robison came to study phosphatase after describing a new phosphate ester. " The hypothesis of Robison48 regarding the necessity of phosphatase for bone formation (which some workers 231 regard as a misconception) was based on such an assumption. The discovery of alkaline phosphatase in the proximal convoluted tubule of the kidney, 191,214 the intestinal microvilli,213 the follicular epithelium of the ovary, 232 and the syncytiotrophoblast of the placenta 233 all suggested some role in the active transport of substances across membranes.

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